【摘要】 介绍了绿色木霉N6 和黑曲霉 85 6原生质体融合子代的研究。经传代、发酵、筛选 ,从 11株初筛的融合子中得到了 3株纤维素酶活高且稳定的菌株AT2 3、AT16、AT34,其CMC酶活分别为亲本绿色木霉N6 的 2 2倍、1 4倍、1 2倍。并对其进行制霉菌素抗性试验和可溶性蛋白质凝胶电泳分析鉴定。试验结果证明了AT2 3、AT16、AT34是基因发生了重组的融合子且具有杂种优势。更多还原

【Abstract】 This paper introduced the study of protoplast fusion progeny betwee Trichoderma viride N₆ and Aspergillus niger 856.By generating,fermenting and screening 3 strains AT23,AT16,AT34 with high cellulase activity and stalle were abtained from 11 strains primary screening fusant.The value of their CMC ase activity is 2.2,1.4,1.2 times respectively of that of N₆.By anti-nystain ability testing and souluable protens SDS-PAGE analyzing identity.The results proved that the 3 strains are gene combination fusion progeny possessing hybridization domination.更多还原