【摘要】 目的是制备特异性的 2 ,4 -D多克隆抗体。用活性酯和混合酸酐法将半抗原 2 ,4 -D分别与牛血清白蛋白 (BSA)和卵清蛋白 (OVA)共价偶联 ;以 2 ,4 -D—BSA作免疫原免疫两只新西兰白兔 ;以 2 ,4 -D—OVA包被 96孔酶标板 ,用间接酶联免疫吸附分析 (ELISA)法检测抗血清效价和竞争性ELISA法检测 2 ,4 -D。结果显示 :2 ,4 -D与BSA和OVA的偶联比率分别为 32∶1和18∶1,抗血清效价 >6 .4× 10 5,在优化条件下 2 ,4 -D的最低检测限达 37ng mL。实验成功地制备了 2 ,4 -D特异性多克隆抗体 ,为其ELISA方法的建立提供了条件。更多还原

【Abstract】 The objective of this paper is to product specific polyclonal antibody of 2,4-D.Hapten 2,4-D was covalently attached to bovine serum albumin (BSA) and ovalbumin (OVA) using the modified active ester method and the mixed-anhydride method, respectively.The 2,4-D-BSA conjugate was used as immunogen to inject two New Zealand white rabbits.With the 2,4-D-OVA conjugate coating 96 well microtiter plates,indirect enzym-linked immunosorbent assay (ELISA) was applied to determine the titer of the antisera and the competitive ELISA was used to detect 2,4-D.The result showed:the titer of the antisera was over 6.4×10 5;The estimated 2,4-D to protein molar ratio was about 32 and 18 for the 2,4-D-BSA and 2,4-D-OVA conjugates,respectively;Under excellent condition, the detection limit of 2,4-D was 37 ng/mL.The specific antibody of 2,4-D was obtained successfully in this experiment and this made it possible to establish ELISA for 2,4-D.更多还原