【摘要】 3-羟基丁酸和3-羟基己酸共聚酯(PHBHHx)是一种新型生物可降解材料,其性能与3-羟基己酸(3HHx)在共聚物中的摩尔百分含量密切相关。本研究在两株嗜水性气单孢菌Aeromonas WQ和Aeromonas hy-drophila 4AK4中分别引入了编码酯酰辅酶A脱氢酶的yafH基因和编码合成3-羟基丁酸-CoA的phbA和phbB基因,将A.hydrophila WQ合成的PHBHHx中的3HHx的摩尔含量由3％-5％提高到20％以上;而A.hydrophila 4AK4合成的PHBHHx中的3HHx摩尔含量则由15％左右降低到3％～12％。成功地实现了对PHBHHx单体组成的调控。更多还原

【Abstract】 Copolyesters consisting of 3 - hydroxybutyrate (3HB) and 3 - hydroxyhexanoate (3HHx) (PHBH-Hx) were synthesized by two recombinant Aeromonas hydrophila strains. The introduction of yafH gene into A . hydrophila WQ led to an increase of 3HHx fraction in PHBHHx from 3 % - 5 % in the wild type strain to over 20% in the recombinant, while foreign genes of phbA and phbB genes in A. hydrophila 4AK4 decreased 3HHx fraction from 15% in the wild type to a desired level of 3% - 12% in the recombinant. Gluconate can also be used to control the 3HHx content in the copolyesters.更多还原