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盐藻线粒体GIY-YIG族归巢内切酶基因受到盐胁迫时增强转录

Transcription of A Mitochondrial GIY-YIG Family Homing Endonucleases Gene Enhanced in Dunaliella salina Under Salt Stress

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【作者】 方孝东林栖凤李冠一屈良鹄

【Author】 FANG Xiao dong 1), 2) , LIN Qi feng 2) , LI Guan yi 2) , QU Liang hu 1) ( 1) The Key Laboratory of Gene Engineering of the Ministry of National Education, Biotechnology Research Center, Zhongshan University, Guangzhou 510275, China; 2) Hainan Provincial Key Laboratory of Biotechnology for Salt tolerance Crops, Institute of Biological Science and Technology, Hainan University, Haikou 570228, China)

【机构】 基因工程教育部重点实验室海南省耐盐作物生物技术重点实验室基因工程教育部重点实验室 中山大学生物工程研究中心广州510275海南大学生物科学技术研究所海口570228中山大学生物工程研究中心

【摘要】 盐藻 (Dunaliellasalina (Chlorophyta) )极强的耐盐能力使之成为研究植物耐盐分子机制的重要模式生物 .在前期分离到的一个盐藻基因片段在盐胁迫时增强表达的基础上 ,通过RACE获得了该基因 5′端cDNA序列及部分 3′端cDNA序列 .序列分析结果表明 ,该基因是一个编码线粒体GIY YIG族归巢内切酶的Ⅰ型内含子基因 .RNase freeDNase处理及Northern杂交结果表明 ,盐藻线粒体DNA在盐处理组中可能发生了扩增 .这些结果结合前人的研究成果说明 ,该内含子基因的增强表达可能并非盐藻对抗盐胁迫的一种手段 ,而是盐藻对抗盐胁迫的副产品 .被该内含子基因插入的基因是盐藻对抗盐胁迫所需要增强表达的 .线粒体DNA的扩增可能是细胞在受到盐胁迫时线粒体数量增加的结果

【Abstract】 Outstanding salt tolerance ability has made Dunaliella salina (Chlorophyta) an important model organism for clarifying the molecular mechanisms of salt tolerance. An isolated Dunaliella salina gene fragment up expressed under salt stress was studied further. 5′ end cDNA sequence and part 3′ end cDNA sequence were obtained through RACE reaction. The sequence analysis showed that this sequence represented a mitochondrial group I intronic gene coding for GIY YIG family homing endonucleases. Combination of RNase free DNase digestion with Northern blot analysis showed that mitochondrial DNA of Dunaliella salina might be amplified under salt stress. These results suggested that the up expression of this intronic gene might be a by product of the process of salt tolerance, which was unnecessary for the salt tolerance of Dunaliella salina , and that the gene containing this intronic gene might be the genuine gene that needed to be up expressed for salt tolerance of Dunaliella salina . The amplification of mitochondrial DNA might be the result of increasing mitochondria under salt stress.

【基金】 国家教育部重点项目(0 2 165 );海南省自然科学基金(3 0 0 0 9);国家“九五”重点科技攻关项目(85 72 2 2 7 0 1);国家科技部项目基金(J0 0 A 0 0 9)资助项目~~
  • 【文献出处】 中国生物化学与分子生物学报 ,Chinese Journal of Biochemistry and Molecular Biology , 编辑部邮箱 ,2003年05期
  • 【分类号】Q943
  • 【被引频次】8
  • 【下载频次】189
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