【摘要】 以少根根霉 (Rhizopusarrhizus)脂肪酶为催化剂 ,有机溶剂为反应介质 ,合成了 3种短链脂肪酸酯 .研究了反应温度、溶剂、底物浓度、底物摩尔比、吸水剂用量等因素对酯化反应的影响 .确定了3种酯的最佳合成条件 :(1)己酸乙酯 :反应温度为 4 0℃ ,环己烷为溶剂 ,0 2 5mol L底物浓度 ,酸醇摩尔比为 1∶1 2 ;(2 )乙酸异丙酯 :5 0℃ ,环己烷为溶剂 ,0 15mol L底物浓度 ,摩尔比为 1∶1;(3)乙酸异戊酯 :5 0℃ ,异辛烷为溶剂 ,0 2 0mol L底物浓度 ,摩尔比为 1∶1.三种酯合成时均需 0 12 5g ml的0 5nm分子筛为吸水剂 ,在 8h后 ,合成酯转化率达到 97%～ 99% .更多还原

【Abstract】 The Rhizopus arrhizus lipase catalyzed syntheses of three short chain esters in organic solvents were studied. The optimum conditions for each esterification were determined as follows: (1)ethyl caproate: synthesized in cyclohexane at 40℃ with 0 25 mol/L substrate and 1∶1 2 acid alcohol molar ratio; (2)isopropyl acetate: reacted in cyclohexane at 50℃ with 0 15 mol/L substrate and 1∶1 molar ratio; (3)isoamyl acetate: in isooctane at 50℃ with 0 20 mol/L substrate, and 1∶1 molar ratio. 0 125 g per ml of 0 5 nm molecular sieve was required as adsorbent for water in the reactions. Under the optimal conditions, the yields reached 97%—99% after incubation for 8 hours.更多还原