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载脂蛋白E3的克隆及原核表达
The Cloning of Apolipoprotein E3 Gene and Its Expression in E.coli
【Abstract】 The apoE cDNA was amplified from four months old fetus liver tissue mRNA by RT PCR. It was inserted into the TOPO ○R vector, whose sequence is the same as that reported. The right apoE cDNA was inserted into pQE30 vector to construct pQE30 apoE recombinant expressed plasmid, which was expressed as a histidine tag fusion protein in E.coli strain BL21(DE3) with higher level. The purified protein was gained by Ni NTA column. SDS PAGE showed that 34 kD protein was expressed. Western blot analysis showed a positive band at 34 kD.
【关键词】 human apoE 3 cDNA;
molecular cloning;
gene expression;
【Key words】 human apoE 3 cDNA; molecular cloning; gene expression;
【Key words】 human apoE 3 cDNA; molecular cloning; gene expression;
- 【文献出处】 中国生物化学与分子生物学报 ,Chinese Journal of Biochemistry and Molecular Biology , 编辑部邮箱 ,2003年01期
- 【分类号】Q78
- 【下载频次】90