【摘要】 蛋白质定向进化在蛋白质工程中取得了令人瞩目的成就 ,其核心技术———随机突变库构建技术已成为近年来体外定向进化研究的热点。在概述定向进化基本原理基础上 ,对几种随机突变技术进行了介绍、分类和比较 ,并对突变库的特征及构建策略予以分析和描述更多还原

【Abstract】 The directed evolution has been applied successfully in the field of protein engineering.Its key technique through constructing gene library by random mutation has recently become a focus in the directed evolution area.we summarized the principle of directed evolution and introduced several random mutation techniques and classified them according to their mutation principles.Characteristics of mutant libraries were compared and strategy of library construction was also discussed.;更多还原