【摘要】 转座子标签 (transposontagging)技术是研究功能基因的有效的工具之一。介绍了几种在酿酒酵母 (Saccharomycescerevisiae)基因功能研究中应用的转座子标签 :mTn3标签、mini Mu噬菌体标签和Ty转座子标签 ,阐述了转座子标签的构建原理、应用策略和转座子标签插入位点的鉴定方法。更多还原

【Abstract】 Transposon tagging is one of the effective technology for genes function analysis .Here,several main transposon taggings including mTn3、mini Mu and Ty,which were applied to exploring the function of yeast genome,were introduced.The principle of transposon taggings and the strategies to identify the site where the taggings insert were summarized.更多还原