【摘要】 蛋白质翻译产物在成熟过程中剪切释放出来的一段氨基酸序列称为“intein”———即内含肽。它与前体蛋白以框内融合的形式共同翻译 ,并内嵌于前体蛋白序列中。内含肽的解离以及内含肽两侧氨基酸序列的连接是在内含肽自身催化作用下完成的。本文将从内含肽的发现、结构特征和作用机理等方面对这种具有特殊意义的蛋白质成熟机制进行较为全面的论述 ,同时介绍了近年来发展起来的以内含肽介导的蛋白质剪接为基础的蛋白质纯化和改造技术。更多还原

【Abstract】 Intein is an intervening protein sequence which could be excised from a protein precursor and flanking protein fragments (the exteins) to form a mature extein host protein and the free intein.This maturing procedure is catalyzed by intein itself and results in a native peptide bond between the ligated exteins.In this review,we would describe the research history of intein,the structure characteristics and its splicing mechanism.At the same time,we would also introduce some protein purification and modification techniques,which were developed recently based on protein splicing. ;更多还原