【摘要】 通过对 2 0株粉拟青霉 (Paecilomycesfarinosus)ITS1- 5 .8S -ITS2 (rDNA)区域序列测定 ,确定了粉拟青霉ITS序列 ,而韩国学者测定的粉拟青霉ITS序列应为细脚拟青霉 (P .tenuipes)的序列。序列比较发现 ,韩国 2株未定名的拟青霉 (Paecilomycesspp .)菌株 (KACC4 0 2 19、KACC4 0 2 2 1)应为粉拟青霉。基于本研究构建的邻接树推断 ,粉拟青霉的有性型可能是一种虫草。粉拟青霉的起源应为单源的。不同的粉拟青霉菌株的ITS序列具有多态性 ,源于同一地区的菌株的ITS变异也较大。ITS序列的证据表明 ,粉拟青霉菌株间的差异与地理来源及寄主均无相关性更多还原

【Abstract】 nrDNA ITS(ITS1 5.8S ITS2) regions of 20 isolates of Paecilomyces farinosus were sequenced, and its correct ITS sequence was obtained. The results showed that the ITS of P. farinosus reported by Korean researchers was actually that of P. tenuipes . Two unidentified Korean isolates of Paecilomyces (KACC40219 and KACC40221) were identified as P. farinosus based on molecular data. The neighbour joining tree showed that P. farinosus is monophyletic, and the teleomorph of P. farinosus was tentatively identified as a Cordyceps species.Sequence polymorphisms of the nrDNA ITS region exist among different isolates of P. farinosus with the same geographic origins. The molecular data proved that genetic diversity of different isolates of P. farinosus is not related to geographic origins and hosts.更多还原