【摘要】 从海生超微型黄藻品系PP983中分离得到一种水溶性色素蛋白复合物 ,其光学特性与普遍存在于甲藻叶绿体中的多甲藻素 叶绿素a 蛋白复合物 (Peridinin Chlorophylla ProteinComplexes,PCP)类似 ;初步测得该色素蛋白复合体的分子量约为 15 4,2 0 0 ,分子中多甲藻素、叶绿素a、蛋白的摩尔比为 16∶2∶5。研究结果显示 ,PP983中的PCP是作为聚光色素复合体存在于藻细胞的叶绿体中的。更多还原

【Abstract】 A water-soluble photosynthetic light-harvesting complex was being isolated from a marine picoalga strain PP983 of Xanthophyta, grown under mass culture conditions. A crude preparation of this complex was obtained as a brick red supernatant by sonication of a whole cell suspension in 0 1 mol L -1 Tris buffer pH8 4 followed by high speed centrifugation. The extract was purified by 30% and 45%(NH 4) 2SO 4 fractionation and Sephadex G-100 chromatography. The pigment-protein complex is characterized by its absorption spectrum having main absorption maxima at 458nm, 482nm and 670nm, and another maximum at approximately 300 nm by using a SHIMADZU UV-1601 spectrophotometer to scan room spectra ranging from 300 to 750nm. The results of absorption spectra showed that the complex have the characteristics of the peridinin-chlorophyll a-protein complex(PCP) of dinoflagellates. Chromophore analysis, by spectral analysis of extracts and polyamide thin-layer chromatography confirmed the presence of peridinin and chlorophyll a in the complex. Therefore, the peak at 458nm and 670 nm corresponds to Soret band and α band of chlorophyll a respectively, and the maximum at 482 nm is due to peridinin present in the complex. The chromoprotein has a molecular weight of about 154, 200 and shows that a peridinin∶chlorophyll a∶protein ratio is close to 16∶2∶5 according to spectrophotometric analyses of chromophore content. The result indicated that the peridinin-chlorophyll a-protein complex presumably functions in vivo as photosynthetic lightharvesting pigments.更多还原