【摘要】 利用硼酸能与3 氯 1,2 丙二醇(3 MCPD)上的2个邻位羟基进行键合生成配合阴离子、从而增加多羟基化合物的负电荷的特性,在15mmol/L三羟甲基氨基甲烷(Tris) 10mmol/LH3BO3(pH8 3)介质中,采用毛细管电泳/电导检测法分离检测了3 MCPD,该方法的线性范围为0 5～20mg/L,检出限为0 1mg/L(S/N=3)。对缓冲溶液的种类、浓度、pH值以及硼酸与3 MCPD的相互作用对分离检测的影响作了探讨。应用于水解植物蛋白(HVP)中的3 MCPD的分离检测,取得了满意结果。更多还原

【Abstract】 3Monochloro1,2propanediol(3MCPD) was the carcinogen existed commonly in hydrolyzed vegetableprotein (HVP) as condiments. Based on the characteristic that boracic acid (H3BO3) has a condasation and complexation reaction with 3MCPD forming a negativecharged adduct in aqueous phase, a rapid method for the direct determination of 3MCPD in HVP by capillary electrophoresis with conductivity detection was investigated. The compounding, pH and concentration of running buffer were investigated to optimize the separation efficiency. Welldefined resolution of 3MCPD in HVP within 12 min was achieved under the conditions of uncoated capillary (50 cm×75 μm id), 15 mmol/L Tris10 mmol/L H3BO3 (pH 83) as running buffer and separation voltage of 18 kV. The linear range was 05-20 mg/L with the detection limit of 01 mg/L. The average recoveries of added standards were 94%-102% according to the results of three samples of HVP. It demonstrates that the proposed method can be applied to the fast determination of 3MCPD in HVP.更多还原