【摘要】 血清和脑脊液样品经甲酯化处理,氯仿提取,氮气吹干,残留物用庚烷溶解后进样,用气相色谱法测定线粒体脑肌病患者血清和脑脊液中乳酸含量。结果显示,线粒体脑肌病患者的血清、脑脊液中乳酸含量分别为(294±101)mg/L和(312±103)mg/L,比正常人((192±37)mg/L和(153±81)mg/L)以及神经系统其他疾病患者((150±34)mg/L和(173±29)mg/L)显著增高(P<0.05)。该方法可作为筛选线粒体脑肌病的临床诊断方法。更多还原

【Abstract】 A novel gas chromatographic method for the determination of lactic acid in serum and cerebrospinal fluid (CSF) of patients with mitochondrial encephalomyopathy has been developed. The serum and CSF samples were methylated with methanol/sulfuric acid, extracted with CHCl3 and dried with N2. Subsequently the residues were dissolved in heptane for chromatographic analysis. The results obtained for serum and CSF were (294±101) mg/L and (312±103) mg/L, respectively, which were remarkably higher than that of control samples ((192±37) mg/L and (153±81) mg/L) and other patients with neural system diseases (serum (150±34) mg/L, CSF(173±29) mg/L). This method is sensitive and rapid, and suitable for screening of mitochondrial encephalomyopathy in clinics.更多还原