【摘要】 以十六烷基三甲基溴化铵(CTAB)/正丁醇/正辛烷/水微乳液作为一种新的展开剂,对23种氨基酸进行了薄层色谱分析。研究了微乳液含水量和氨基酸分子结构对Rf值的影响;同时选择含水量为40%的微乳液对氨基酸混合物进行了分离鉴定并与传统的展开剂作了比较;此外还探讨了微乳色谱理论。研究结果表明,当微乳液为油包水型及油水双连续型时,氨基酸的Rf值多在0.2与0.8之间,色谱分析结果令人满意。更多还原

【Abstract】 The chromatographic behaviors of 23 amino acids on the silica gel thin layers using cetyltrimethylammonium bromide(CTAB)/nbutyl alcohol/noctane/water microemulsions as a developer has been studied. The effects of hydrous content of microemulsions and structures of amino acids on the Rf values were investigated. Several amino acid mixtures were separated and determined using a microemulsion with 40% hydrous content which was compared with the traditional developer of ethanol/water/acetic acid mixture. The chromatographic mechanism of microemulsion was also discussed. The results show that the waterinoil (W/O) microemulsion and the bicontinuous microemulsion composed of the mentioned components appeared to be the suitable developers for thin layer chromatographic analysis for the amino acids.更多还原