【摘要】 试验者为小细胞肺癌患者,于右上臂皮下植入缓释VP 16(又称依托泊苷或鬼臼乙叉苷),按预定时间点采集静脉血并分离出血浆。血浆样品以氯仿萃取,常温下负压挥发至干,然后以流动相溶解,采用反相高效液相色谱法测定。流动相为V(甲醇)∶V(水)=52∶48的溶液,检测波长为UV 228nm,色谱柱为LichrosphereC18(250mm×4 6mmi d ,5μm)。用外标法定量。实验结果表明,该测定方法的检出限为0 02mg/L,绝对回收率为80 4%,相对回收率为94 7%,日内和日间精密度(用相对标准偏差RSD表示)均为8%左右;VP 16与血浆中其他成分(内源性干扰物)达到良好分离。此外还进行了血浆样品的稳定性试验,发现VP 16的萃后残渣于-5～0℃可保存240h。方法灵敏度较高,准确性和实用性好,为临床应用提供了可靠的检测方法。更多还原

【Abstract】 The patients with cellule lung cancer were implanted sustained releaseetoposide (VP16) on the right upper arms. The plasma was separated from the vein blood collected at prearranged times from these patents. The plasma sample was extracted with chloroform, and evaporated at ordinary temperature under reduced pressure. Then it was dissolved by the mobile phase, and determined by external standard reversedphase high performance liquid chromatography with a Lichrosphere C18 (250 mm×46 mm id,5 μm) using methanolwater (10∶90 in volume ratio) as the mobile phase at the detection wavelength of UV228 nm. The detection limit was 002 mg/L. The absolute recovery was 804%. The relative recovery was 947%. The RSDs of intraday and interday were about 8%. With the method VP16 was well separated from the other components in the plasma. The sample could be preserved as residue in dry state after pretreatment at -50 ℃ for 240 h . This method is reliable, accurate and sensitive, and can be used in practical clinics.更多还原