大豆中β-伴球蛋白的分离与纯化

【摘要】 从大豆中分离制备β-伴球蛋白,对通过电泳进行分析鉴定。采用分级盐析(70%～90%)和凝胶过滤(Sepharose 6B)分离纯化大豆中的β-伴球蛋白。免疫电泳的结果证实所提取的β-伴球蛋白纯度很高,大豆中的其它成分与β-伴球蛋白抗血清无交叉免疫反应。SDS-PAGE的结果表明本实验所提取的β-伴球蛋白是由不同形式单体组成的复合物,共有6种亚基。更多还原

【Abstract】 conglycinin in soybean was purified, and then assayed by electrophoresis. β- conglycinin in soybean wasprepared by salt precipitate (70%～90%) and gel filtration on a Sepharise 6B column. The result of immunoelectrophoresis illustratedthat the purified β- conglycinin was not contaminated,while the antiserum of β- conglycinin showed no cross-reaction withthe other proteins in soybean. The prepared β- conglycinin was heterogeneous based on SDS-PAGE, and was composed of sixkinds of subunits.更多还原