再生丝素蛋白固定过氧化氢酶传感器的研究

【摘要】 从莴苣种子中提取过氧化氢粗酶,利用再生丝素蛋白在甲醇作用下,其分子结构由可溶性randomcoil向不溶性β-sheet发生转变,从而将过氧化氢粗酶固定在的β-sheet所特有的分子氢键中,制得过氧化氢传感器。该传感器在pH=6.5的KH2PO4-NaOH工作介质中,过氧化氢浓度在5.0×10-5～3.0×10-3mol/L范围内呈良好线性关系。更多还原

【Abstract】 Catalase lixiviated from lettuce seed was immobilized in regenerated silk fibroin membrane.Because of the structuraltransition of the silk fibroin from random coil to antiparallel β-sheet induced by methanol treatment,the biosensor of catalasehad a linear response over the range 5.0×10-5 ～3.0×10-3mol/L in KH2PO4-NaOH buffer(pH=6.5).更多还原