【摘要】 研究了以壳聚糖膜为载体、戊二醛为交联剂的固定化葡萄糖氧化酶的特性。结果表明:固定化酶、溶液酶的最适pH均为5.5;固定化酶、溶液酶的最适温度分别为40℃,35℃;固定化酶的Km值为12.16mmol/L,溶液酶的Km值为20.86mmol/L;固定化酶比溶液酶更耐热,且贮藏稳定性及操作稳定性也有所提高;该固定化酶重复使用率较高。更多还原

【Abstract】 The propertie of glucose oxidase covalently immobilized on glutarald e h yde-treated chitosan membrane change.The optimum pH value for Immobilized glu co se oxidase (IGOD) and glucose oxidase(GOD)are 5.5;The optimum temperature fo r I GOD and GOD were 40℃ and 35℃,respectively. The apparent Km of IGOD is 12. 06 mmol/L while Km of GOD is 20.86mmol/L:the thermal stability of IGOD is im prov ed by immobilizati on. The immobilized enzyme had good storage and operatio n st ability and reusability and that properties offer potential for further pra ctica l application.更多还原