【摘要】 在食品样品中加入体积分数 60 %高氯酸溶液去蛋白 ,过滤后进样检测叶酸。流动相选用pH 3 .5 ,体积分数 12 %的乙腈 ,浓度 5 0mmol/L的KH2 PO4溶液。流速为 1mL/min ,经ODSC 18柱分离 (柱温为 40℃ ) ,用质量分数 0 .5 %的过二硫酸钾溶液作柱后衍生剂 ,流速为 0 .3mL/min ,柱后反应温度为 60℃ ,经荧光检测器 (Ex :3 65nm ,Em :45 0nm)检测定量。实验结果表明 ,在 0 0 1～3 2 μg/mL的浓度范围内 ,线性良好 ,相关系数为 0 .9999;最低检出浓度为 0 .0 1μg/mL ;方法回收率为 98.1% ;变异系数为 2 3 %。更多还原

【Abstract】 perchloric aicd was added into food sample to remove protein. After filtering the sample, the filtrate was injected into HPLC to measure its folic acid. 50mmol/L potassium dihydrogen phosphate containing 12% acetonitrile (v/v), pH 3.5 was used as mobile phase, the flow rate was 1mL/min. ODS C18 was used as separating column, column temperature was 40℃. Potassium persulfate was chosen as postcolumn derivative agent, the flow rate was 0.3 mL/min, reaction temperature was 60℃. The folic acid derivate was measured with fluorescence detector (Ex:365nm, Em: 450nm). It is seen that the standard curve was linear with the range of 0.01～3.2μm/mL, its related coefficient was 0.9999. The determination limit of this method was 0.01ng/mL, the yield was 98.1% and the variation coefficient was 2.3%.更多还原