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壳聚糖凝胶固定葡萄糖氧化酶制备酶电极的工艺
Study on the Process of Preparing Glucose Oxidase Electrode with Chitosan Gel as Immobilization Matrix
【摘要】 以壳聚糖作为固定GOD载体 ,研究了壳聚糖溶液的粘度 ,交联剂戊二醛的浓度、用量以及铂丝在酶膜母液中浸涂时间等对葡萄糖传感性能的影响。在优化固定化条件的基础上 ,建立了通过调整固定化条件来适应载体溶液粘度变化的工艺 ,制备出性能基本一致的酶电极
【Abstract】 With glucose oxidase immobilization in chitosan matrix,the effect of the viscosity of the chitosan solution,the concentration and amount of gluatraldehyde solution,and the dipping time of the platinum wire in enzyme membrane solution on performance of glucose sensors were investigated On the basis of the optimum parametrers immobilized by GOD,by adjust immobilization parametrers to adapt to the viscosity changes of chitosan solution,the technology was formulated and the glucose sensor with approximate performance was prepared
【Key words】 chitosan; degradation; glucose oxidase; immobilize; enzyme electrode;
- 【文献出处】 食品与发酵工业 ,Food and Fermentation Industries , 编辑部邮箱 ,2003年08期
- 【分类号】O646
- 【被引频次】10
- 【下载频次】303