【摘要】 在 72只麻醉大鼠 ,分别采用后肢、肾脏和肠系膜动脉在体恒流灌注法 ,观察了向灌流环路中直接注射植物雌激素三羟异黄酮 (genistein ,GST)的血管效应 ,以所引起的灌流压增减反映血管的收缩和舒张。结果如下 :( 1)不同剂量的GST ( 0 4、0 8、1 2mg/kg)注射于股部灌注环路时 ,剂量依赖性地降低股动脉的灌流压。GST的这一效应可被L 硝基精氨酸甲酯 (L NAME)部分阻断 ,预先注射蛋白酪氨酸磷酸酶抑制剂正钒酸钠 ( 5 0 μg/kg) ,可部分抑制GST( 0 8mg/kg)引起的效应 ;( 2 )向肾血管灌注环路中直接注射GST也可剂量依赖性地降低肾动脉的灌流压 ,预先注射正钒酸钠可完全抑制GST引起的效应 ,而L NAME对此效应没有影响 ;( 3 )肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压 ,这一效应可被正钒酸钠部分抑制 ,而L NAME对此无影响。根据上述结果得出的结论是 :GST降低后肢、肾脏和肠系膜血管床的血管张力 ,其机制与酪氨酸激酶抑制有关 ,而在股动脉则与NO释放有部分关系更多还原

【Abstract】 The effects of local injection of genistein on femoral, renal, and mesenteric vascular beds were investigated respectively by constant flow perfusion method in 72 anaesthetized rats. The results are as follows: (1) genistein (0.4, 0.8, 1.2 mg/kg) decreased the perfusion pressure (PP) of femoral vascular bed in a dose-dependent manner. The effect of genistein (0.8 mg/kg) was partially inhibited by L-NAME, or by sodium orthovanadate (50 μg/kg), a potent inhibitor of protein tyrosine phosphatase; (2) genistein also decreased the PP of renal vascular bed in a dose-dependent manner and the effect of genistein was completely inhibited by pretreatment with sodium orthovanadate, but unaffected by L-NAME; and (3) genistein decreased the PP of mesenteric vascular bed in a dose-dependent manner, an effect which was partially inhibited by sodium orthovanadate, but unaffected by L-NAME. From the results obtained, it is concluded that genistein can decrease the vascular tone in the femoral, renal, and mesenteric vascular beds with the underlying mechanism that involves tyrosine kinase inhibition, while in femoral arterial beds, it also involves NO release.更多还原