【摘要】 目的　研究碱性成纤维细胞生长因子 (bFGF)和血管内皮生长因子 (VEGF)在大鼠创伤脑组织中的表达及它们之间的关系 ,从分子水平探讨颅脑损伤后的病理机制 ,为临床治疗脑损伤的新途径提供实验基础。方法　改进Marmarou大鼠加速弥漫性脑损伤模型 ,制作成弥漫性轴索损伤同时合并局灶性脑挫伤的新的动物模型 ,取挫伤灶周围脑组织免疫组化染色观察bFGF、VEGF基因表达情况。结果　脑挫伤灶周围脑组织bFGF基因表达在伤后 1h明显增加 ,伤后 12h达高峰 ;VEGF基因表达伤后逐渐增加 ,2 4h达高峰。结论　bFGF、VEGF基因表达与脑损伤密切相关 ,bFGF、VEGF作为生长因子可能参与颅脑损伤后神经元保护及损伤后修复过程。更多还原

【Abstract】 Objective To study the expression and interrelationship of bFGF and VEGF at different time in a rat model of traumatic brain injury (TBI), and to investigate the pathogenic mechanism of brain injury at molecular level. Methods Diffuse axonal injury with local contusion was produced in Wistar rats by the method described by Marmarou with some modifications. The expressions of bFGF and VEGF of brain around contusion area were observed by immunohistochemical methods. Results The expression of bFGF mRNA began to increase 1 h postinjury, and reached the maximum level in 12 h in marginal area of injury focus. The expression of VEGF mRNA increased following brain injury and peaked in 24 h postinjury in marginal area of injury focus. Conclusion The results showed a close relationship between bFGF?VEGF gene expression and the degree of brain injury. As growth factors, bFGF and VEGF might play a role in neuronal protection and repair following traumatic brain injury .更多还原