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胃肠道癌患者外周血CD8~+T细胞功能相关标志物的检测及意义

Significance of measurement of the peripheral CD8~+T cell function-related makers in patients with gastrointestinal carcinoma

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【作者】 沈立松董平吴政宏徐翀沈定丰李莉全志伟

【Author】 SHEN Lisong, DONG Ping, WU Zhenghong, XU Chong, SHEN Dingfeng, LI Li, QUAN Zhiwei. Xin Hua Hospital and Shanghai Children’s Medical Center, Shanghai 200127, China

【机构】 上海第二医科大学附属新华医院上海儿童医学中心实验诊断中心上海第二医科大学附属新华医院成人外科上海第二医科大学附属新华医院成人外科 上海200127上海200092上海200127上海200092

【摘要】 目的 探讨胃肠道癌患者外周血T细胞亚群和CD8+ T细胞CD2 8共刺激分子等功能相关性标志物的表达及意义。方法 用三色或四色荧光标记流式细胞术检测 2 7例胃肠道癌患者及 16例正常对照的外周血淋巴细胞。结果 与正常对照组相比 ,肿瘤患者外周血T细胞标志如CD3+ 、CD4 + 、CD8+ 表达水平及CD4 + /CD8+ 比值差异无显著性 ;CD8+ CD2 8-T细胞显著增加 (P <0 .0 1) ,而CD8+ CD2 8+ T细胞显著降低 (P <0 .0 5 )。表达HLA DR的活化CD8+ T细胞显著增加 (P <0 .0 1) ,分泌IFN γ的CD8+ T细胞 (Tc1)明显增多 (P <0 .0 1) ,而分泌IL 4的CD8+ T细胞 (Tc2 )则正常人和肿瘤患者均很低。患者CD8+ T细胞CD2 8及IFN γ的表达变化与肿瘤分期无关。结论 胃肠道癌患者中CD8+ T细胞存在系统性活化的表现 ,其机制可能与CD2 8/B7以外的信号传递有关。胃肠道癌患者体内Tc1细胞的增加与肿瘤的分期缺乏明确关系。

【Abstract】 Objective To investigate the significance of CD8 +T lymphocyte function-related makers and changes of T lymphocyte subsets in patients with gastrointestinal carcinoma. Methods Subjects for this investigation include 27 patients with gastrointestinal carcinoma and 16 healthy controls. Peripheral T lymphocyte subsets, CD28, HLA-DR and the production of IFN-γ and IL-4 in CD8 + T cells were analyzed by flow cytometry. Results There was no significant difference between cancer patients and healthy controls in peripheral T lymphocytes in CD3 +, CD4 +, CD8 + expressions as well as CD4/CD8 ratio. The CD8 + CD28 -(Ts) subpopulation in T cells was significantly increased in cancer patients( P <0.01); The CD8 +CD28 +(CTL) subpopulation in T cells was significantly decreased in cancer patients( P <0.05). The expression of HLA-DR on CD8 +T cells was much higher in cancer patients than that in healthy controls( P <0.01), and a significant increase of IFN-γ producing CD8 +T cell population(Tc1) was noticed in cancer patients( P <0.01), the percentage of IL-4 producing T cells(Tc2) in both healthy controls and patients was very low. Conclusions The phenomena of the systemic activation of CD8 +T cells in gastrointestinal carcinoma may be resulted from the mechanisms other than CD28/B7 signal transduction pathways. The increase of Tc1 cells in gastrointestinal carcinoma has no clear relation with the staging of tumor.

  • 【文献出处】 上海医学检验杂志 ,Shanghai Journal of Medical Laboratory Sciences , 编辑部邮箱 ,2003年04期
  • 【分类号】R735
  • 【被引频次】16
  • 【下载频次】145
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