【摘要】 目的　探讨胃肠道癌患者外周血T细胞亚群和CD8+ T细胞CD2 8共刺激分子等功能相关性标志物的表达及意义。方法　用三色或四色荧光标记流式细胞术检测 2 7例胃肠道癌患者及 16例正常对照的外周血淋巴细胞。结果　与正常对照组相比 ,肿瘤患者外周血T细胞标志如CD3+ 、CD4 + 、CD8+ 表达水平及CD4 + /CD8+ 比值差异无显著性 ;CD8+ CD2 8-T细胞显著增加 (P <0 .0 1) ,而CD8+ CD2 8+ T细胞显著降低 (P <0 .0 5 )。表达HLA DR的活化CD8+ T细胞显著增加 (P <0 .0 1) ,分泌IFN γ的CD8+ T细胞 (Tc1)明显增多 (P <0 .0 1) ,而分泌IL 4的CD8+ T细胞 (Tc2 )则正常人和肿瘤患者均很低。患者CD8+ T细胞CD2 8及IFN γ的表达变化与肿瘤分期无关。结论　胃肠道癌患者中CD8+ T细胞存在系统性活化的表现 ,其机制可能与CD2 8/B7以外的信号传递有关。胃肠道癌患者体内Tc1细胞的增加与肿瘤的分期缺乏明确关系。更多还原

【Abstract】 Objective To investigate the significance of CD8 +T lymphocyte function-related makers and changes of T lymphocyte subsets in patients with gastrointestinal carcinoma. Methods Subjects for this investigation include 27 patients with gastrointestinal carcinoma and 16 healthy controls. Peripheral T lymphocyte subsets, CD28, HLA-DR and the production of IFN-γ and IL-4 in CD8 + T cells were analyzed by flow cytometry. Results There was no significant difference between cancer patients and healthy controls in peripheral T lymphocytes in CD3 +, CD4 +, CD8 + expressions as well as CD4/CD8 ratio. The CD8 + CD28 -(Ts) subpopulation in T cells was significantly increased in cancer patients( P <0.01); The CD8 +CD28 +(CTL) subpopulation in T cells was significantly decreased in cancer patients( P <0.05). The expression of HLA-DR on CD8 +T cells was much higher in cancer patients than that in healthy controls( P <0.01), and a significant increase of IFN-γ producing CD8 +T cell population(Tc1) was noticed in cancer patients( P <0.01), the percentage of IL-4 producing T cells(Tc2) in both healthy controls and patients was very low. Conclusions The phenomena of the systemic activation of CD8 +T cells in gastrointestinal carcinoma may be resulted from the mechanisms other than CD28/B7 signal transduction pathways. The increase of Tc1 cells in gastrointestinal carcinoma has no clear relation with the staging of tumor.更多还原