【摘要】 目的　探讨大肠癌中胎盘型谷胱甘肽 S 转移酶 (GST Pi)、拓朴异构酶 Ⅱ (TOPO Ⅱ )和P 糖蛋白(PgP)耐药基因的表达及生物学意义。方法　采用即用型免疫组织化学微波加热法 ,对 98例大肠癌进行GST Pi、TOPO Ⅱ和PgP耐药基因检测分析。结果　① 98例大肠癌中的GST Pi、TOPO Ⅱ和PgP的阳性表达率分别为 45 .92 %、2 6.5 3 %和 11.2 2 %。②大肠癌中GST Pi的阳性表达与年龄呈正相关 (r =0 .92 ,P <0 .0 0 5 )。结论　部分大肠癌患者的体内存在多向耐药基因 ,或癌细胞内原发存在对GST Pi、TOPO Ⅱ和PgP所介导的抗癌药物的耐药基因。对大肠癌患者在化疗前进行耐药基因检测 ,有助于选择合理的联合用药 ,提高疗效 ,延长患者的生存期。更多还原

【Abstract】 Objective To probe into the expression and biological significance of resistance gene as Glutathione S Transferase pi(GST Pi), TopoisomeraseⅡ(TOPO Ⅱ), P glycoprotein(PgP) in large bowel carcinoma. Methods To test and analyze the resistance gene GST Pi, TOPO Ⅱ, PgP in 98 cases of large bowel carcinoma by instant microwave heating immunohistochemistry. Results ①Positive expression rates of GST Pi, TOPO Ⅱ, PgP in 98 cases of large bowel carcinoma were 45.92% (45/98),26.53% (26/98) and 11.22% (11/98), respectively. ②There were some correlations between the postive expression of GST Pi in large bowel carcinoma and age of the patients. Conclusion There are presence of multidrug resistance genes in some patients of large bowel carcinoma or resistance to GST Pi, TOPO Ⅱ, PgP existed in the cancer cell primarily. Before starting the chemotherapy, determination of the above resistance gene may be helpful for clinicians to select a rational regime and dosage to improve the curative effect and prolong the life span.更多还原