【摘要】 目的 研究绿茶对人乳腺癌细胞及人脐静脉内皮细胞碱性成纤维细胞生长因子(bFGF)表达的影响及机制。方法 ELISA法检测bFGF的蛋白表达水平;mRNA的水平用Northern印迹杂交法研究。结果 40μg/mL的绿茶提取物(green tea extract,GTE)或绿茶主要有效成分表没食子儿茶素-3-没食子酸(epigallocatechin-3gallste,EGCG)①能够抑制人脐静脉内皮细胞及人乳腺癌细胞株细胞内及细胞分泌入条件培养基中bFGF多肽水平,这种抑制作用呈剂量依赖性;②能明显抑制MDA-MB231细胞内的bFGF reRAN表达水平。结论 GTE或EGCG能够在mRNA和蛋白水平抑制乳腺癌细胞及脐静脉内皮细胞的bFGF表达,绿茶可能通过该机制抑制肿瘤新生血管形成。更多还原

【Abstract】 Purpose To explore the role of green tea on basic fibroblast growth factor (bFGF) in human breast cancer cells and human umbilical vein endothelial cells (HUVECs) and its mechanisms. Methods bFGF concentrations were determined by ELISA. Northern blot hybridization was performed to detect the expression of mRNA. Results 40 μg/mL GTE or EGCG could decrease the levels of bFGF peptide secreted into conditioned media as well as the bFGF peptide levels in both HUVECs and human breast cancer cells; this effect was dose dependent. 40 μg/mL GTE and EGCG decreased the mRNA levels of bFGF in MDA-MB231 cells. Conclusions Green tea can inhibit bFGF expression in human breast cancer cells and human umbilical vein endothelial cells through multiple levels. This may be the partial mechanism for green tea to inhibit angiogenesis in cancers.更多还原