【摘要】 目的　探讨超声辐照能否加快骨组织电镜样品的脱钙过程。方法　固定后的样品浸入含 134mmol/L和NaOH 70mmol/L的脱钙液中 ,经 2 1kHz的超声波辐照 ,每天 2 0min共 7d。未经超声波处理的样品在脱钙液中浸泡 2 0d作为对照。在透射电镜下观察两组骨组织的超微结构。结果　经超声辐照的样品在骨基质中没有无机盐结晶残留 ,且超微结构保存良好 ,与对照组无差异。结论　超声辐照可使脱钙处理的时间大大缩短 ,仅为对照组的三分之一更多还原

【Abstract】 Purpose To explore whether ultrasonic irradiation can shorten the process of osseous tissue decalcification during the sample preparation for electron microscopy. Methods The fixed samples immersed in the decalcifying solution containing disodium ethylene diamino tetraacetic acid (EDTA·Na 2)134 mmol/L and Naoh 70 mmol/L were irradiated by ultrasonic waves at a frequency of 21 kHz for 20 min per day.The irradiation continued for 7 days.The samples immersed in the same decalcifying solution for 20 days without using the ultrasonic irradiation were used as the control.The ultrastructure of the osseous tissue in the two groups were observed under transmission electron microscope. Results In the sections irradiated by the ultrasonic waves,there were no deposit of inorganic salt crystals shown on bone matrices,and the ultrastructure was preserved well.It seemed no difference from that of the control. Conclusions Ultrasonic irradiation can shorten the decalcifying period.It was reduced to one third of the control in this study.更多还原