【摘要】 发根农杆菌 (Agrobacteriumrhizogenes)R16 0 1感染商陆叶片外植体 1周后 ,在其切口处产生毛状根 ,2 0d后产生毛状根的外植体比例达 70 % ;毛状根可直接从叶片外植体叶脉处或从叶脉处产生的愈伤组织上产生。毛状根能在无激素的MS培养基上自主生长 ,其呼吸速率比对照根提高 85 6 %。冠瘿碱检测和PCR扩增结果表明 ,发根农杆菌RiT DNA的冠瘿碱合成酶基因及其Ri质粒的rol基因均已在商陆毛状根基因组中得到表达。毛状根中总皂甙含量约为自然根的 1 5 4倍 ,但其多糖含量则仅为非转化根的 70 %。更多还原

【Abstract】 Hairy roots appeared in vitro 20 days after inoculation of Phytolacca esculenta leaf explants with the strain of Agrobacterium rhizogenes R1601 The frequency of leaf explants transformed by R1601 was up to 70% Hairy roots can be incited directly from the veins of explants or via callus Hairy roots induced by R1601 grew rapidly on medium MS without hormone and were 85 6% higher in respiration rate than control roots Transformation was confirmed by opine detection and the amplification of rol B and rol C genes from the hairy roots of P esculenta The total saponin content in hairy roots of P esculenta was about 1 54 times as much as in natural roots whereas polysaccharides content was about 70% times as much as in the natural roots更多还原