【摘要】 清道夫受体能结合带负电荷的大分子 ,特别是经过修饰的脂蛋白颗粒 ,与血浆脂质代谢有密切关系。为研究A类清道夫受体的过量表达对血脂代谢的影响 ,首先从小鼠巨噬细胞中经RT PCR获得鼠清道夫受体A类I型 (MSR AI)的表达序列 ,插入到含人清道夫受体基因增强子和金属硫蛋白启动子的真核表达载体中。选择Km×ICR杂交一代为实验小鼠 ,显微注射、移卵后 ,获得转基因小鼠 ,经PCR、Southern杂交鉴定 ,获得完整整合外源DNA序列的首建鼠 4只 ,拷贝数分别为 15、6、2和 4。Northern杂交实验显示A类清道夫受体基因主要在脾脏中表达 ;在金属锌诱导下 ,MSR AI表达有所增高 ,同时血清中甘油三酯水平显著升高 ,胆固醇浓度下降。结果表明A类清道夫受体的过量表达能显著影响血脂水平 ,具体机制需进一步研究。更多还原

【Abstract】 Scavenger receptor (SR) is characterized by its ability to bind negatively charged macromolecules, particularly the modified lipoproteins that are pertinent to the development of vascular disease. To determine the role of excessive scavenger receptor A in the serum lipoprotein metabolism, transgenic mice lines with mouse scavenger receptor A gene type I (SR-AI) under the control of human SR-AI enhancer and metallothionein gene promotor were established. After zinc induction, the expression of SR-AI in transgenic mice was a little higher than the controls, but the serum lipids levels were significantly different from the controls, especially the cholesterol. These results demonstrated that overexpression of SR-AI significantly affected the serum lipids levels.更多还原