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高碱性pH诱导紫膜片层表面结构变化的原子力显微镜直接观测
The Direct Observation on The Surface Structure Change of Purple Membrane Induced by High pH Using Atom Force Microscope
【摘要】 报道了在高碱性pH下 ,紫膜中细菌视紫红质 (BR)表面结构变化的直观信息 .紫外可见光谱实验发现 ,当pH上升到 12 6 ,BR分子上的生色团视黄醛脱落 ,分子完全变性 ;原子力显微镜实验观测到在此pH下 ,紫膜片层的晶格结构瓦解 ,BR分子在紫膜上无规则聚集 ,同时出现非特征“岛屿”结构和特征“岛屿”结构
【Abstract】 The direct information of the surface structure change of purple membrane under high pH was obtained. The UV VIS spectra showed that BR was totally denatured and lost its retinal under the pH 12 6. It was found by atom force microscope that the crystalline lattice of purple membrane was broken down at this pH. The typical and non typical “Island” structures emerged as the BR molecules assemble irregularly in purple membrane.
【关键词】 细菌视紫红质;
紫膜;
pH;
“岛屿”结构;
原子力显微镜;
【Key words】 bacteriorhodopsin; purple membrane; pH; “Island” structure; atom force microscope;
【Key words】 bacteriorhodopsin; purple membrane; pH; “Island” structure; atom force microscope;
【基金】 国家自然科学基金资助项目 ( 3 0 170 2 3 5 ) ;中国科学院重大项目(KJCX1 SW );国家重点基础研究发展规划资助项目 ( 973 )(G19980 1 0 10 2 )~~
- 【文献出处】 生物化学与生物物理进展 ,Progress In Biochemistry and Biophysics , 编辑部邮箱 ,2003年05期
- 【分类号】Q936
- 【被引频次】6
- 【下载频次】67