【摘要】 在已有的研究基础上 ,优化和建立了单个植入前胚胎的mRNA差异显示 (singlepreimplantationembryodifferentialdisplaypolymerasechainraction ,SPEDDRT PCR) .以小鼠单个成熟的卵母细胞和单个的 2细胞期胚胎作为起始材料 ,比较二者之间基因的表达差异 .选择在卵母细胞中不表达而在 2细胞期表达的差异片段 .进行GenBank检索和表达序列标签 (EST)库电子克隆 .与多胚研究方法一样 ,由线粒体编码的和 2细胞特异表达的NADH脱氢酶亚单位 2和ATPase 6证实了SPEDDRT PCR方法是可行而且可靠的 ,是一种需要材料极少、应用广泛而有效的基因分离方法更多还原

【Abstract】 Based on previous studies, mRNA differential display using a single mouse oocyte or a 2 cell embryo as starting materials was established. cDNA fragments, which were expressed in 2 cell stage mouse embryos but not in MⅡ stage, were performed computer cloning and GenBank search. Two genes, NADH dehydrogenase gene subunit 2 and ATPase 6,which encoded by mitochondrial genome, were found to be stage specifical expressed in 2 cell stage pre implantation embryos. These results, which were the same as multi embryonic mRNA differential display, strongly suggest that single preimplantation embryos differential display polymerase chain reaction (SPEDDRT PCR) is a credible, practical and effective method that needs only a single embryo.更多还原