【摘要】 为了研究B7 1分子功能性mAb (6H2 )对表达B7 1分子的B系淋巴瘤Raji细胞的生物效应。分别运用免疫荧光标记和流式细胞仪技术 (FACS )、台盼蓝拒染试验、MTT试验和RT PCR半定量分析 ,分析 6H2对Raji细胞的生长抑制和凋亡诱导作用。结果显示 ,6H2能抑制天然表达B7 1分子的人B系淋巴瘤Raji细胞的生长 ,产生细胞周期G2 /M的阻滞 ,上调细胞表面CD18和CD95 (Fas )的表达 ,且能使Raji细胞中Bax的相对转录表达显著增加 ,从而诱导Raji细胞的凋亡。 6H2为功能性抗人B7 1分子特异性mAb ,具有抑制Raji细胞生长和凋亡诱导作用 ,在B系淋巴瘤研究和生物治疗中具有应用价值。更多还原

【Abstract】 To study the biological effects of the functional monoclonal antibody(6H2)against B7 1 molecule on B lymphoma cell line Raji,the effects of 6H2 on the growth inhibition and apoptosis induction on Raji cells were investigated by means of immunofluorescence staining,FACS analysis,trypan blue exclusion,MTT assay and RT PCR It was found that the 6H2 could inhibit the growth of Raji cells that naturally expressed human B7 1 molecule In addition,6H2 also blocked the cell cycle G2/M and up regulated the surface expression of CD18 and CD95(Fas).Beside these,it also induce the marked increase of comparative transcription of Bax and apoptosis of Raji cells It concludes that 6H2 can inhibit the growth of Raji cells and induce apoptosis of these cells and it may be expected to have a potential value in the therapeutic approach in the management of B lymphoma更多还原