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蝴蝶兰不同花色品种遗传多样性的RAPD分析

RAPD ANALYSIS ON GENETIC DIVERSITIES OF Phalaenopsis VARIETIES WITH DIFFERENT FLOWER COLORS

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【Author】 Ming Feng 1) Dong Yuguang 2) Lou Yuxia 3) Wang Jingwen 1)Liang Bin 1) Ye Mingming 1) Shen Daling 1)*( 1)Institute of Genetics, State Key Laboratory of Genetic Engineering,Research Center of Gene Diversity and Designed Agriculture,Ministry of Education Key Laboratory for Biodiversity Science andEcological Engineering, School of Life Science, Fudan University, Shanghai 200433; 2)Shanghai Animal and Plant Introduction Research Center,Shanghai Academy of Agricultural Sciences, Shanghai 201106; 3)Biotechnology Lab, Shanghai Teachers University, Shanghai 201418)

【机构】复旦大学生命科学院；遗传学研究所；遗传工程国家重点实验室；基因多样性与设计农业中心；生物多样性与生态工程教育部重点实验室；上海市农业科学院动植物引种研究中心；上海师范大学生物工程实验室；生物多样性与生态工程教育部重点实验室上海200433；上海201106；上海201418；上海200433；上海200433；

【摘要】利用筛选到的 2 5个特异引物对蝴蝶兰不同花色品种作RAPD分析 ,确定了 12个品种间的遗传距离 ;并根据Neighbor joining遗传距离矩阵构建系统聚类文件 ,将该 12个品种分为两个家族 :第一家族中包含基色为白色的花色品种 ,花蕊颜色略有不同 ;第二家族为具有条纹的花色品种。品种间遗传距离最大为 0 .783 2 ,最小为 0 .10 3 4。更多还原

【Abstract】 Genetic distance between 12 varieties of Phalaenopsis was determined through RAPD with 25 specific random primers. The 12 Phalaenopsis breeds were divided into two families according to the Neighbor-joining clustering analysis. In the first family, there were seven varieties having the same key color-white color and slightly different stamen colors; and in the other family, the flowers of the varieties had steaks. The longest genetic distance between varieties was 0.7832, and the shortest was 0.1034.更多还原

【关键词】 RAPD；蝴蝶兰；花色；家族；
【Key words】 RAPD； Phalaenopsis； Flower color； Families；

【基金】上海市科学技术发展基金资助课题 (项目编号 :0 13912 0 0 7)

【文献出处】上海农业学报 ,Acta Agriculturae Shanghai , 编辑部邮箱 ,2003年02期

【分类号】S682.31
【被引频次】69
【下载频次】439

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