【摘要】 目的探讨血管内膜增生(IH)过程中膜型基质金属蛋白酶(MT-MMP)mRNA的表达与平滑肌细胞(SMC)迁移的关系。 方法建立大鼠血管IH模型,以原位杂交法检测术后血管壁中MT-MMP mRNA的表达,并以特殊染色及Desmin免疫组化法对SMC进行半定量测定,观察两者的相关性。 结果IH过程中,MT-MMP mRNA与Desmin表达之间存在一定的相关性。术后前4周呈正相关(r=0.933,P<0.05),而此后表现为负相关(r=-0.42,P=NS)。 结论IH早期(术后1月),MT-MMP mRNA过度表达,激活MMPs,介导SMC由中膜向内膜迁移。更多还原

【Abstract】 Objective To explore the relationship between the mRNA expression of membrane type matrix metalloproteinase ( MT - MMP) , an activator of matrix metalloproteinases zymogen (pro - MMPs) , and smooth muscle cells ( SMC) migration in the development of vascular intimal hyperplasia ( IH ). Methods We established a model of rat autologous vein graft in the carotid artery, and investigated the expression of MT - MMP mRNA using ire situ hybridization. SMC was measured using special staining and im-munohistochemical assays. Results Positive correlation was found between the expression of MT - MMP mRNA and SMC migration during the first four weeks of IH (r = 0. 933, P <0. 05) , while negative correlation was found in the rest time of IH ( r = -0. 42, P = NS). Conclusion We suggest that in the early stage of IH , MT - MMP mRNA overexpresses and causes degradation of extracellular matrix ( ECM ) by activating pro -MMPs, which eventually enable the medial SMC migrate to the intima.更多还原