【摘要】 利用RAPD技术分析了我国沿海辽宁、江苏、广西省3个文蛤地理群的遗传变异,总共用60个随机引物进行了RAPD扩增,其中50个随机引物得到了扩增产物,选取其中15个随机引物的扩增图谱进行相似性系数和遗传距离的计算,结果为辽宁(LW)、江苏(JW)、广西(GW)群体内的遗传距离分别为:0 169±0 034,0 117±0 032,0 108±0 031.LW,JW之间为0 222±0 036;JW,GW之间为0 209±0 043;LW,GW之间为0 316±0 047,同时发现引物s396-900bp的标记为JW和LW所特有;引物s433-250bp标记为JW和GW所特有,由此可作为这3个地理群的分子标记.更多还原

【Abstract】 Genetic variation of three wild stocks of Meretrix meretrix was analysed using random amplified polymorphic DNA(RAPD)markers,50 primers got amplified bands in total of 60 primers,amplified bands of 15 primers were selected as statistic similarity index and genetic distances. Genetic distances of intra-stock of Liaonin, Jiangsu Province and the Zhuang Autonomous Region of Guangxi are 0.169±0.034,0.117±0.032 and 0.108±0.031,respectively;and those of inter-stock of Liaonin-Jiangsu, Jiangsu-Guangxi and Guangxi-Liaonin are 0.222±(0.036),0.209±0.013 and 0.316±0.047,respectively,the results show that genetic distances of three stock increase with distance increase. The marker of s396-900 bp is owned by Jiangsu and Liaonin stocks,and marker of s433-250 bp is owned by Jiangsu and Guangxi stocks,three stocks were indentified by this two markers.更多还原