【摘要】 目的 研究成人骨髓间充质干细胞（hMSC）体外定向诱导可否分化为神经元样细胞。方法Percoll分离液离心分离hMSC,体外扩增,采用两种方法:①含碱性成纤维细胞生长因子（bFGF）预诱导24小时,甲氧酚（BHA）和二甲亚枫（DMSO）联合诱导6小时;②2-巯基乙醇（2-ME）预诱导24小时,BHA和DMSO诱导6小时。免疫组化检测神经元样细胞表达神经元烯醇化酶（NSE）、神经丝蛋白（NF）、胶质纤维酸性蛋白（GFAP）。结果 hMSC在体外扩增传至5代后,流式细胞仪显示hMSC表面抗原CD₂₉、CD₄₄、CD₉₀表达阳性率分别为99.5％,97.8％,98.8％。采用两种方法诱导hMSC后,胞体收缩,突起伸出,较密的部分神经元拉成网状;免疫组化显示诱导的神经元样细胞能特异性表达NSE、NF,不表达GFAP。结论 成人骨髓hMSC在体外可以分化为神经元样细胞。更多还原

【Abstract】 Objective To investigate the differentiation from human mesenchymal stem cells (hMSC) into neuron-like cells. Methods hMSC were isolated by percoll separating medium from rib marrow aud expanded in culture medium. The surface antigen expression of hMSC was detected by flow cytometry. hMSC were induced to differentiate into neuron-like cells with bFGF.DMSO and HBA or 2-ME,DMSO and HBA respectively. Specific markers and structures were detected by immunohistochemistry and histochemistry. Results hMSC were expanded to be undifferentiated cells in culture for more than 5 passages. Flow cytometry showed that 99. 5%, 97. 8%, 98-8% hMSC express strongly antigens CD29,CD44,CD90 which was identical to human bone marrow-derived MSC. Immunohistochemistry staining showed that the induced different-passage hMSC expressed neuron-specific enolase(NSE),neurofilament(NF),but did not express glial fibrillary acidic protein(GFAP). Conclusion hMSC can be differentiated into neurons in vitro.更多还原