【摘要】 运用MTT比色实验测定细胞增生能力,通过球体细胞培养、细胞生长曲线的测定以及细胞形态结构的观察,研究抗癌药莪棱舌草Ⅰ号对人肝癌细胞(7721细胞)的抑制作用。结果:莪棱舌草Ⅰ号使7721细胞的增生能力明显下降(抑制率高达70％以上),细胞球体明显减小甚至解体,细胞形态明显改变。这种抑制作用可能是莪棱舌草Ⅰ号对肝癌细胞产生杀伤作用的机制之一。更多还原

【Abstract】 TCSs are compounds of anticancer medicine, including three kinds of Chinese traditional herbs. The TCSs effect of inhibition on human hepatoma cell line 7721 was determined by adopting the following four methods: MTT colorimetry experiment, multicellular tumor spheroid(MTS) , measure of the cell’s growth curve, and observation of the tumor cell’s configuration. The results indicated that the herb obviously lowered proliferation of the tumor cell with the inhibitory rate as high as 70 %. Furthermore, the herb efficiently diminished the volume of the cell’s globe or disjoint it. Finally, the cell’s configuration was changed greatly by the herb. These inhibitory effects probably infer one of the mechanisms of the killing effect of TCSs on hepatoma cells.更多还原