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缺血预处理的次数对兔缺血心肌电生理活动的影响

Study on effect of various dose of ischemia preconditioning on rabbit cardia celectrophysiological activity

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【作者】 王蓉曾晓荣陈连璧

【Author】 WANG Rong,ZENG Xiao-Rong,CHEN Lian-Bi (Dept.of Physiology,School of M edicine,Shandong University)

【机构】 山东大学医学院生理研究所泸州医学院心肌电生理研究室山东大学医学院生理研究所

【摘要】 目的:观察缺血预处理(IPC)的次数对缺血-再灌流过程中兔心脏电生理活动的影响,探讨IPC的抗心律失常作用机制。方法:家兔24只,随机分为对照组(C)、缺血组(IR)、一次预处理组(SPC)和两次预处理组(BPC)。应用心外膜单相动作电位(MAP)探针记录技术检测缺血-再灌流过程中MAP幅度(MAPA)、复极50%时程(MAPD50)、零相最大上升速度(Vmax)及心律失常发生率。结果:各预处理组中缺血后5min时的MAPD50、MAPA、Vmax迅速缩短或降低(P<0.05)。SPC组与IR组相比,促进了缺血20min之后MAPA、Vmax的恢复,改善了缺血20minMAPD50的过度缩短,限制了复灌时MAPD50的过度延长(P<0.05)。SPC完全抑制了缺血-再灌中室颤的发生率(0%,P<0.05)。BPC组中,缺血-再灌注中室颤发生率及在缺血30min末及再灌注末上述参数与缺血组比较均无显著差异。结论:①SPC在在体家兔心脏上具有最好的抗心律失常作用,随IPC次数增加,其抗心律失常作并不增强;②缺血早期SPC迅速缩短MAPD,抗早期心律失常的机制可能是缩小了造成单向阻滞的窗口;③改善缺血晚期电生理参数,抗晚期心律失常的机制可能是减小不应期离散度;④限制复灌时MAPD过度延长,抗再灌注心律失常可能与减少内向离子流及触发活性有关。

【Abstract】 Objective:To investigate the effects o f various“doses”of ischemia preconditioning(IPC)on electrophysiological activi ty in acute ischemia heart of rabbit and to approach the possible antiarrhythmic mechanism of IPC.Methods:Twenty-four rabbits were divided into four groups random-ly:control,IR(ischemia-reperfusion),SPC(sin gle ischemia precond itioning)and BPC(bi-ischemia precon-ditioning).With the recording techniqu es of monophasic action potential(MAP),we continually monitored MAP to obers erve the effects of various“dose”of IPC on parameters of MAP such as(50%of dura-tion of MAP)MAPD 50 ,(amplitude of MAP)MAPA,and(the maxium depolarization velocity)Vmax an d the incidence of arrhythmia.the ECG was monitored in experiment.Re sults:At 5min after ischemia com-pared with those of IR group,the MAPD 50 ,MAPA and Vmax in SPC and BPC groups were significant-ly decreased(P<0 .05)and improved at20-min after ischemia in SPC group;in addition.the e xcessive width of MAPD 50 in reperfusion was limited in SPC group(P<0.05).SPC totally suppress ed ventricular fibrillation(P<0.05).The incidence of arrhythmia at the e nd of ischemia in BPC group was similar to those in IR group.Conclusions:①SPC is the best selection to acquire the a ntiarrhthymic effect that has no dose-dependency.②In the early phase of is ch emia SPC promotes shortening of MAPD 50 so as to weaken slow conduction.③In the late phase of ischemia SPC improve s parameters of MAP so as to re-duce the dispersion of(effective refractory pe riod)ERP.④In reperfusion SPC limits the excessive width of MAPD 50 .

  • 【文献出处】 山东大学学报(医学版) ,Acta Academiae Medicinae Shandong , 编辑部邮箱 ,2003年06期
  • 【分类号】R363
  • 【下载频次】57
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