【摘要】 目的:建立人胆囊癌裸鼠皮下瘤模型,并对其生物学特性进行初步观察。方法:培养人胆囊癌细胞系GBC-SD细胞,收集细胞并制备细胞悬液。每只裸鼠于股外侧皮下注射GBC-SD细胞悬液0.2ml(约2×106个细胞),共30只。观察GBC-SD细胞系成瘤率、成瘤时间、肿瘤生长情况及对裸鼠机体状况的影响。解剖观察瘤体情况并行病理切片观察。结果:裸鼠成瘤率为100%,GBC-SD细胞系对高周龄裸鼠亦有致瘤性,成瘤时间为1周左右。裸鼠于注射瘤细胞后约8周出现恶液质,晚期恶液质明显并衰竭死亡。荷瘤生存期平均为20周。结论:GBC-SD人胆囊癌裸鼠皮下瘤模型成瘤率高,GBC-SD细胞系对高周龄裸鼠亦有致瘤性。肿瘤生长速度稳定,易于观察,可作为胆囊癌治疗等研究的良好平台。更多还原

【Abstract】 Objective:To establish a novel subcutaneous tumor model in nude mice by using hu-man gallbladder carcinoma cell line GBC-SD and observe its biological characteristics.Methods:GBC-SD cell line was cultured and the cultured cells were harvested to make cell suspension.Each of30mice was injected0.2ml GBC-SD cell suspension(2×10 6 cells)subcutaneously into femoral outside.Then tumor forma-tion rate,tumor appearing time,growing characteristics and its effects on general condition of the nude mice were observed,and anatomical and pathological examination was performed as well.Results:The overall tumor formation rate was100%,and the average tumor appearing time was1week after tumor cell suspension injection.Cachexia occurred in about the8th week,and the average life-span of the mice was about 20weeks.Con clusion:The human gallbladder carcinoma subcutaneous tumor model was suc-cessfully established and can now be used in the researches concerning gallbladder carcinoma treatment.更多还原