【摘要】 目的:构建HPV16L1-E7C重组质粒并研究其细胞免疫性。方法:利用基因克隆技术将HPV16L1-E7CDNA片段定向插入pcDNA3.1载体,构建重组质粒pcDNA16L1-E7C,然后将其免疫C57BL/6小鼠,利用T淋巴细胞增殖试验检测免疫小鼠的脾淋巴细胞增殖能力,同时利用ELISA试验检测免疫小鼠的脾淋巴细胞分泌γ-干扰素(IFN-γ)的水平。结果:特异性T细胞增殖试验中实验组3H-TdR掺入率为18339±1972,对照组为6737±1243,实验组小鼠T细胞增殖水平明显高于对照组(P<0.05)。DNA免疫后实验组小鼠脾淋巴细胞分泌IFN-γ水平为0.89±0.56,对照组为4.81±1.33,两者相比差异有统计学意义(P<0.05)。结论:重组质粒能增强小鼠的细胞免疫功能。更多还原

【Abstract】 Objective:To construct the recombinant plasmid of HPV16L1-E7C and study its cel-lular immunity.Methods:The HPV16L1-E7C DNA fragment was inserted into the pcDNA3.1vector by gene cloning.Then the plasmid pcDNAL1-E7C was used to immunize female C57BL/6mice three times at2weeks intervals.T lymphocyte proliferation test and ELISA were used to detect the specific cellular immunity of recombinant plasmid.Result:In the T cell proliferation test,the 3 H-TdR incorpo-ration in the experimental group was18339±1972and6737±1243in the control group,showing a higher in the experimental group than in the control group(P<0.05).After DNA immunization,the se-cretion level of IFN-γin the experimental group was0.89±0.56and that in the control group was4.81±1.33,with a statislically significant difference(P<0.05).Conclusion:The recombinant plasmid could significantly enhance the cellular immunity of mice.更多还原