【摘要】 为了解甲壳动物胚胎发育过程中主要营养物质消耗利用的规律,采用生物化学方法测定了日本沼虾胚胎发育过程中7个发育期的主要生化成分。结果表明,在日本沼虾胚胎发育过程中,水分含量在早期无显著变化,发育至溞状幼体期达到最高。蛋白质含量在早期略有升高,而从后无节幼体晚期开始下降。脂类含量则逐期下降,只是在前溞状幼体期有所升高。这表明在胚胎发育过程中,脂类和蛋白质不仅在构建组织器官过程中起到重要作用,且两者可能分别作为胚胎发育不同时期的主要能量来源。脂肪酸变化研究表明:DHA主要参与细胞和亚细胞结构形成。亚油酸和亚麻酸的含量在胚胎发育过程中下降,可能为胚胎发育提供能量,并合成部分DHA。∑_ω-3HUFA在前溞状幼体期含量开始增加,主要用于胚胎发育后期细胞结构的形成。棕榈油酸在溞状幼体期含量骤然下降,主要作为能量物质被消耗。日本沼虾胚胎发育不同阶段主要生化成分的变化与其组织、器官的发育关系密切。更多还原

【Abstract】 To study the metabolism of the main nutrition during embryonic development of the Crustacea, the variations in biochemical composition during embryonic development of Macrobrachium nipponense were determined by biochemical methods. The results showed that water content had a little change in the early stage of embryonic development, but reached the highest content at the zoea stage. Protein content increased in the early stage and began to decrease from the late stage of egg-metanauplius. The content of lipid decreased with the embryonic development stages and increased at the stage of egg-protozoea. All those indicated that protein and lipid were the main energy sources for the embryonic development of M. nipponense, and they also were the important sources for tissue differentiation and organization. Based on the variation of fatty acid composition, DHA participated in tissue differentiation and organization. The linoleinc acid and linoleic acid offered energy for embryonic development, at the same time they synthesized DHA. ∑ω-3HUFA was concerned with the forming of cells in the late stage of embryonic development. The palmitoleic acid was probably concerned with energy metabolizing. The conclusion is that the variations in biochemical composition during embryonic development of M. nipponense closely correlate with the development of the tissue and organization.更多还原