【摘要】 目的　建立简单、快捷、费用低廉的糖检测方法 ,用微量化显色反应检测糖代谢。方法　将临床用糖检测试剂盒配套用校准液作为样本 ,缩小反应体积测定在不同葡萄糖含量 ,相同反应体积 ,或相同葡萄糖含量 ,不同反应体积时 ,OD值与葡萄糖的含量关系 ,并用此法测定在胰岛素作用下 3T3 L1脂肪细胞和HepG2细胞的糖代谢。 结果　反应体积缩小后 ,OD值仍与葡萄糖的含量成正比 ,且体积对OD值影响甚微 ,并能反映胰岛素促靶细胞 3T3 L1脂肪细胞和HepG2细胞对糖的摄取利用特性。 结论　GOD POD微量化测试法的建立是成功的 ,且简单、快捷、费用低廉。此法不仅能用于体外培养细胞的糖代谢检测 ,也能用于与糖代谢有关的药物筛选 ,值得推广利用更多还原

【Abstract】 Objective In vitro, to establish an easy,shortcut, cheap method with GOD-POD assay in a minimal wayto detect glucose. MethodIn order to study the relationship between OD and glucose content, calibration liquid in clinical reagent is used as sample to detect glucose in two minimal ways. One is to add different glucose content in identical volume, another is to add same glucose in different volume .and again, medium containing insulin ,in which 3T3-L1 adipocyte and HepG2 cell was cutured anddetected in that minimal way.Results OD isdirectly proportional to glucose content,but it is almost not affected by reactivevolume.Conclusion GOD-POD assay in a minimal way is an easy,shortcut, cheap method to detect the glucose, and this method can be used not only to detect glucose metabolism of cells, but also to screen drugs associated with glucose metabolism.Thus, it is a good way to be spreaded and utilized.更多还原