【摘要】 将透明颤菌(Vitreoscilla)血红蛋白基因(vgb)插入几丁质酶基因表达质粒中,然后转入大肠杆菌(Escherichiacoli)内,研究vgb基因的表达对几丁质酶基因表达和产物分泌的影响.结果表明,在不同的通氧条件下,vgb基因的表达产物均能不同程度地促进细胞的生长,而且发酵上清液的几丁质酶活性也有提高;若溶氧浓度越低,则效果越明显,可比对照提高达12.1%.同时,由于vgb在大肠杆菌的表达,还可进一步促进几丁质酶在大肠杆菌中的分泌.更多还原

【Abstract】 A recombinant plasmid pCV23 was constructed by inserting the Vitreoscilla Hemoglobin (VHb) gene into the plasmid pCHI, which contained the chitinase gene cloned from Bacillus circulans C2. To study the influence of VHb to cell growth and chitinase production, both plasmids were transformed into Escherichia coli JM109, respectively. The results showed that the expression of VHb could improve cell growth in different dissolved oxygen concentrations. And the production of chitinase was enhanced with 12.1% higher than that of control. It was observed that VHb also influenced the chitinase secretion.更多还原