【摘要】 首先对其他物种cbr/elip基因的同源序列进行相似性分析,设计一对简并引物,利用TR PCR技术获得一250bp的片段,经克隆测序分析发现其同D.bardwail的cbr基因有67.0%的同源性,然后再以此片段为模板设计引物,通过RACE技术构建盐生杜氏藻cbr基因的全长序列.更多还原

【Abstract】 At first, the degenerate primers were designed based on the homologous gene of cbr/elip in other organisms. Then, using RTPCR technique, the authors obtained a cDNA fragment of 250bp from D.salina. After cloning and DNA sequencing, the result indicated that 67.0% of the sequence of the fragment was homologous to the cbr in D.bardwail. Based on this sequence, the authors newly designed a pairs of primers. By the RACE technique, the fulllength cDNA of cbr was successfully constructed in D.salina.更多还原