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HPLC法柱后衍生测定乳制品中牛磺酸的含量
Determination of taurine in dairy by HPLC derivation after column
【摘要】 建立了高效液相色谱法测定乳制品中牛磺酸含量的方法。采用荧光检测器,柱后OPA衍生剂衍生,色谱柱为Waerts氨基酸分析柱,柱温为25℃,流动相流速为0.4 mL/min,衍生剂流速为0.4 mL/min。标准曲线相关系数为0.9749;回收率为95.6%;8次样品测定SD为0.383 2,变异系数为1.2136%。
【Abstract】 A method for determination of taurine in dairy by HPLC is reported. Fluorscence detector, Waters-amino acid analysis column and derivation after column was used with column ternperature 25℃ and speed of mobile phase 0. 4 mL/ min and agent of derivation 0. 4 mL/min. The relative coefficient was 0. 9749, the recovery 95. 6% ; the SD in 8 sample by testing was 0. 3832 and CV= 1.2136%.
- 【文献出处】 中国乳品工业 ,China Dairy Industry , 编辑部邮箱 ,2003年04期
- 【分类号】TS252.7
- 【被引频次】16
- 【下载频次】220