【摘要】 目的 :研究胃腺癌及其配对淋巴结转移灶中上皮性钙黏素 (E cd)、p2 7蛋白和增殖细胞核抗原(PCNA)的表达及其意义。方法 :应用免疫组织化学S P法检测 2 0例正常胃黏膜 ,2 0例不典型增生、10 9例胃腺癌原发灶及相应淋巴结转移灶中E cd、p2 7和PCNA表达。结果 :E cd、p2 7和PCNA在不典型增生、胃腺癌原发灶及淋巴结转移灶中阳性表达率分别为 85 % (17 2 0 )、4 0 37% (4 4 10 9)、2 3 85 % (2 6 10 9)、6 0 % (12 2 0 )、33 0 3% (36 10 9)、19 2 7% (2 1 10 9)和 2 5 % (5 2 0 )、74 31% (81 10 9)及 77 98% (85 10 9) ,三者之间差异有显著意义 ,P <0 0 1。E cd和p2 7表达与胃腺Lauren分型、TNM分期和预后显著相关。E cd和p2 7的下降主要见于弥漫型胃癌 ,其在淋巴结转移灶中的表达明显低于原发灶。结论 :E cd、p2 7和PCNA可反映胃腺癌的生物学特性 ,是有价值的预后指标更多还原

【Abstract】 Objective To study the expression and prognostic value of E-cadherin(E-cd),p27 and proliferating cell nuclear antigen (PCNA) in gastric adenocarcinoma and their lymph-node metastases.Methods Immunohistochemical method was used to detect E-cd,p27 protein and PCNA expression in 20 normal gastric mucosa tissues,20 gastric epithelial dysplasia,109 advanced gastric adenocarcinomas and matched lymph-node metastases.Results The positive expression rates of E-cd,p27 protein and PCNA in gastric epithelial dysplasia,gastric adenocarcinomas and matched lymph-node metastases were 85%(17/20),40.37%(44/109),23.85%(26/109),60%(12/20),33.03%(36/109),19.27%(21/109) and 25%(5/20),74.31%(81/109),77.98%(85/109) respectively with significant difference,P<0.01.The positive expressions of E-cd and p27 were closely related to Lauren type,TNM categories,and prognosis.The expression of PCNA was closely correlated with growth pattern,Lauren type,blood vessel invasion,and prognosis.The expressions of E-cd and p27 were closely linked to diffuse-type gastric adenocarcinomas.Comparing the primary cancers with the matched lymph-node metastases,the expressions of E-cd and p27 were reduced.Conclusions E-cd,p27 and PCNA might be valuable markers in assessing biological behavior and prognosis in gastric adenocarcinomas.更多还原