【摘要】 目的 :初步探讨人脐静脉内皮细胞 (HUVEC)原代培养的电离辐射效应。方法 :HUVEC经体外原代培养并用电镜及免疫组化法鉴定 ;形态学观察和流式细胞仪 (FCM )测定单次 6MV X射线照射 0～10Gy后 2 4和 4 8h细胞周期和凋亡 ;放免法和硝酸还原酶法分别测定内皮素 (ET 1)及一氧化氮 (NO)代谢产物NO-2 的含量。结果 :按本法原代培养的HUVEC生长良好 ,并证明是血管内皮细胞 ;FCM经 5和 10Gy照射后 2 4及 4 8h出现明显G0 G1期细胞比例减少 ,G2 M期增加及分泌ET 1减少 ,但无照射引起的细胞凋亡和NO分泌的变化。结论 :本方法原代培养的HUVEC经鉴定确为人血管内皮细胞 ,生长汇合后有一定的辐射抗拒性。更多还原

【Abstract】 Objective To culture primary human umbilical vein endothelial cells in vitro and research its radiation-effect.Methods HUVECs was primary cultured in vitro and identified with electron microscope and immunohistochemical method.Morphology and flow cytometry(FCM) were used to detect changes of cell cycle and apoptosis in primary HUVECs cells irradiated with 6 MV X-ray.Contents of ET-1 and NO were detected with radioimmunoassay and HNO 3 reductase method respectively.Results Primary HUVECs grow very well and was proved to be endothelial cells.Decreases of cells’ rate in G 0/G 1 and contents of ET-1,and a increase in G 2/M phase were detected in irradiated confluent HUVECs;apoptosis and contents of NO were not changed after exposure to X-ray with 0-10 Gy.Conclusions The cultured primary cells was proved to be endothelial cells indeed,confluent HUVECs were partly resistant to radiation.更多还原