【摘要】 目的 :研究老年非小细胞肺癌 (NSCLC)组织中Caspase活化的癌细胞凋亡与临床病理特征的关系。方法 :对手术切除的老年NSCLC组织标本 5 0例 (鳞癌 2 4例 ,腺癌 2 6例 ) ,采用Caspase特异的单克隆抗体—M30CytoDEATH免疫组化染色方法显示凋亡细胞 ,计算凋亡指数 (AI)。结果 :2 8 0 % (14 /5 0 )的肺癌组织标本中可见不同程度的M 30表达。其中肺鳞癌组织的M30阳性表达率 (4 1 7% )明显高于肺腺癌 (15 3% ) ,差异有显著意义 ,P <0 0 5 ;肺鳞癌组织的AI亦高于腺癌 ,差异有显著意义 ,P <0 0 5。低分化NSCLC组织的AI高于中、高分化的NSCLC组织 ,差异有显著意义 ,P <0 0 5 ;低分化鳞癌组织的AI明显高于中、高分化的鳞癌 ,差异有显著意义 ,P <0 0 5。M30的表达与其他临床病理学特征无相关性。结论 :非小细胞肺癌组织中存在Caspase活化的自发性癌细胞凋亡机制 ,且Caspase的表达与组织类型及组织分化程度有关。更多还原

【Abstract】 Objective To observe the correlation of apoptosis with active caspase to the clinical characters of elderly non-small cell lung cancer(NSCLC).Methods M30 CytoDEATH,an antibody specific for the human Caspase family,was used to display apoptosis cells in 50 NSCLC tissues.The apoptosis index (AI) was calculated.Results The expression of M30 in squamous carcinomas(41.7%) was higher than that in adenocarcinomas(15.3%) and the difference was significant.AI in squamous carcinomas was also higher than that in adenocarcinomas and the difference was significant.AI in the poorly differentiated carcinomas was significantly higher than that in the middle- and well-differentiated carcinomas.Conclusion Spontaneous apoptosis cells mainly occur in the tissues of squamous carcinoma and poorly differentiated carcinoma.更多还原