【摘要】 目的 :观察碱性成纤维细胞生长因子 (bFGF)和苏拉明 (suramin)对体外培养人脐静脉内皮细胞生长 (HUVEC)的影响 ,旨在探讨他们在肿瘤血管生成和抗肿瘤血管生成中的作用。方法 :体外培养人脐静脉内皮细胞EV 30 4 ,一组用不同浓度的bFGF(0 0 1、0 1、1 0、10、10 0ng mL)进行培养 ,另一组在用不同浓度的苏拉明 (0 0 1、0 1、1 0、10、10 0 μmol L)培养 6h后加入浓度为 10ng mL的bFGF再进行培养。结果 :bFGF可明显刺激内皮细胞的生长 ,而Suramin可阻止bFGF对内皮细胞的生长刺激。结论 :苏拉明对体外培养内皮细胞的生长抑制作用可能是通过中和肽类生长因子如bFGF及引起内皮细胞凋亡来实现的。更多还原

【Abstract】 Objective To investigate the angiogenic role of bFGF and the antiangiogenic mechanism of suramin.Methods One group of umbilical veinous endothelial cells (HUVEC)EV 304 were cultured with different concentration of bFGF (0 01,0 1,1 0,10,100 ng/mL).Others were cultured with different concentration of suramin (0 01,0 1,1 0,10,100 μmol/L),and after 6 hours stimulated by the concentration of bFGF (10 ng/mL).Results The growth of HUVEC was notably stimulated by bFGF,which was inhibited by suramin.Conclusion The inhibition of suramin for bFGF induced growth of HUVEC is through blocking the stimulation of bFGF and inducing apoptosis of HUVEC. China J Cancer Prev Treat,2003,10(3):289-292更多还原