【摘要】 目的 :探讨Endostatin对裸鼠大肠癌血管内皮生成因子 (VEGF)及其受体 (Flk 1)的抑制作用的机理。方法 :建立大肠癌裸鼠模型 ,将裸鼠分为 2组 ,分别给予Endostatin和生理盐水治疗 ,15d后处死裸鼠 ,采集肿瘤标本 ,免疫组化法检测瘤株的VEGF和Flk 1的表达情况。结果 :Endostatin对VEGF没有抑制作用 (P >0 0 5 ) ,对Flk 1有抑制作用 (P <0 0 5 )。结论 :Endostatin通过竞争性抑制VEGF与其Flk 1结合实现抑制肿瘤生长的目的。更多还原

【Abstract】 Objective To observe the effects and analyse the mechanism of the angiogenesis inhibitor endostatin by curing the nude mice implanted carcinoma of colon cell line.Methods Tumor cells were implanted subcutaneous in the hind flank of female mice of 6 weeks.Animal was treated with Endostatin and Saline daily.The specimen was harvested,and the expression of VEGF and flk 1 were observed with immunohistochemical method.Results 1)Endostatin has no inhibition to the VEGF secreted by the tumor cells.2)By specifically combining with VEGF/flk 1 receptor,Endostatin can refrain the angiogenesis caused by VEGF.Conclusions Endostatin can inhibit the growth of tumor by restraining the angiogenesis of tumor. China J cancer Prev Treat,2003,10(3):253-255更多还原