【摘要】 自然界是微生物的大本营,工业微生物几乎都是从自然界选育来的。我们从兰州市淀粉厂附近的土壤中分离到产异淀粉酶的菌株,将样品于富集培养基中进行富集养后,分离、纯化筛选出酶活较高的菌株,对其进行了鉴定。依据新版伯杰细菌鉴定手册,鉴定该菌株为地衣芽胞杆菌(BacillusLicheniformis),与文献[1,2]中所报道的异淀粉酶菌的产生菌是一致的对该菌株的生长特性进一步研究的结果是2-18小时是该菌的对数生长期,在16小时时菌体生长最快,18小时以后菌体生长进入稳定期。菌体32℃摇瓶发酵培养不同时间后能在540nm处测其OD值计算其酶活发现该菌的产酶是伴随着菌体的生长同时发生的,当菌体生长达稳定期后,产酶量迅速增加,到48小时,产酶量达最多,48小时以后产酶量逐渐趋于稳定。更多还原

【Abstract】 The primitive strain was isolated from the soil of the starch factory in LanZhou.According to its grown character.it was identificated as Bacillicus Licheniformis.The strain grows fastesi at 16 hour After 18hours,it enters steady grown period.with its growing.it also produces isoamylase.After it entering steady period,the amount of isoamylase increase rapidly,To 48 hours,the amount of isoamlase get most.After 48 hours,it tends to steading.更多还原